Xi Chen

5 Questions 3 Answers 0 Followers

Questions related from Xi Chen

Buffer (medium) and cell density for red blood cell (erythrocyte) – based assays?

I’m new to red blood cell-based assays. Anyone can help me answer the following questions? Thank you very much! I plan to evaluate the effect of a compound on the hemolysis of human erythrocytes....

10 October 2022 2,548 0 View

T cell culture problem, big and irregular (elongated) - shaped floating cells?

We cultured antigen - specific CD8+ T cells for 3 passages with IL2 supplement (also stimulated once with CD3/CD28/CD2); we noticed a lot of elongated cells floating (not attached) within the...

11 June 2019 4,047 5 View

Any good protocol/reference to extract cell wall/plasma membrane glycoprotein from actinobacteria?

I would like to extract total glycoprotein within the cell wall/plasma membrane from some actinobacteria (gram positive bacteria). I’m totally new to this field and have very limited knowledge....

09 January 2018 2,071 0 View

How to interpret this cell invasion assay result?

I performed a cell invasion assay to asses invasive capability of two different tumor population by using matrigel coated inserts. After experiment, I found that: No. of stained cells on the...

04 January 2018 731 5 View

Does anybody know what type of cell death is this?

After treating a compound to my cells, I noticed that cells died in a way I have never seen before (see the attached image - panel A). I don't think its apoptosis (apoptosis look like panel B)....

14 October 2017 4,415 3 View