Wing-Hang Tong

2 Questions 2 Answers 0 Followers

Questions related from Wing-Hang Tong

How do you sterilize a poly I:C stock for TLR3 activation?

I used to order a sterilized poly I:C from Calbiochem, but they are out of stock for an indefinite period of time. I ordered lyophilized poly I:C from Sigma (P1530) but it turned out to be not...

02 February 2014 3,798 0 View

For experiments with macrophages, I made a poly I:C stock solution, heated it to 65C and let it cool to RT, and kept aliquots in -20C freezer.

Do I have to heat them to 65C and let it cool to RT before every use?

11 November 2013 5,486 2 View