William Lee

3 Questions 5 Answers 0 Followers

Questions related from William Lee

Why is my 'pKD46' plasmid not cured after Lambda red recombination?

Hi guys! I have a problem with my pKD46(which has heat-sensitive origin pSC101) not cured, after lambda red recombination. I confirmed my recombinant bacmid right after red recombination by PCR...

08 November 2022 323 6 View

What's wrong with my SacB counterselection in Bacmid ?

Hi everyone ! I'm trying to mutate Bacmid (MultiBac™ - Geneva Biotech) by using lambda red recombination. I've Knocked out particular gene in bacmid and replaced with cat-SacB cassette....

16 December 2021 4,854 4 View

Does 'Removing propeptide' renders protein to be secreted out from cell?

Hi guys, I'm trying to express recombinant GPI-Anchor protein(Alkaline phosphatase) in insect cell. As you all know, GPI-Anchor protein has signal peptide for ER-pathway and...

01 January 1970 7,486 0 View