Wei Zhang

9 Questions 6 Answers 0 Followers

Questions related from Wei Zhang

Selection of buffers for viability dye and antibody cocktails in flow cytometry?

I am trying to create a workflow for flow cytometry experiment sample prep. I intend to use LIVE/DEAD™ Fixable Aqua Stain and an antibody cocktail for extracellular antigens. I intend to use the...

12 February 2024 6,499 1 View

Fluorescence beads for practicing flow cytometry technique?

Hi all, I recently started working with flow cytometry with no prior knowledge. I have been trying to stain cells to practice flow cytometry techniques, such as panel setup, gating, and data...

18 December 2023 9,400 2 View

How do fats/fatty acids dissolve in milk?

I'm trying to develop a milk fat quantitation method by spiking fats/fatty acids in skimmed cow milk to around 1.5% fat (w/w). Curious if anyone has experience in this regard. If you have ever...

11 October 2022 1,935 2 View

How does qPCR primer extension end?

So I have been reading the principles of qPCR for the amplification of DNA. So the reaction takes place after the primers have been combined to DNA single strands, and enzymatic reactions elongate...

21 June 2022 9,517 2 View

How to separate solid fractions from solution?

The current work I'm conducting includes an incubation process of small molecules with solid fractions. After the incubation, I usually centrifuge the solids down in a regular centrifuge and it...

07 January 2021 9,058 9 View

How do you determine the pH of mixtures of water and organic solvents?

Dear fellow researchers, I am currently trying to prepare solutions of water mixed with different organic solvents, such as methanol, acetonitrile...But our lab doesn't have proper electrodes on...

05 May 2020 7,459 0 View

What could cause CE-MS signal to decrease with every run in one sequence?

So I am running a sequence analyzing One single standards mixture in positive mode. The mixture is in 0.1% formic acid-containing 95% water/5% acetonitrile and the BGE is 10% acetic acid....

24 September 2018 2,000 1 View

What effect does a high Capillary Voltage (VCap) have on the TOF MS hardware?

I am currently working on a CE-MS system, MS being Agilent 6230. I use the system in the positive mode and the capillary voltage is set at 5500 V. And the system works properly. I'm a bit curious...

06 July 2018 4,672 3 View

How to understand the repeatability of intracellular metabolome analysis?

We know that turnover rates of many metabolites inside cells can be very rapid, and it is important to analyze them at a very fast manner. How would one explain that " the turnover rate of the...

27 October 2016 7,679 1 View