Dear all, is there a difference between 0.1% and 5% trichloroacetic acid (TCA) when used to extract plant leaves... does increase in TCA concentration affects the composition of the extract? Thank you
I think it realy depends on what you want to analyze. For example the methods I know for extracting acid-soluble metabolites from plants normaly use 10-20 % TCA, propably for enzyme-quenching reasons.
This is also the biggest difference, I think, depending on what you want to analyze, you should aim for 5 % TCA and higher for proper quenching.
Kind regards
Henryk Straube Henryk Straube I am extracting hydrogen peroxide and assaying its content with xyleniol orange. 5% extract gives notably higher values compared to 0.1% TCA extract ... so my question was does 5% TCA extract has different metabolites profile? ... thanks
I am not sure whether I get your question right, but a higher concentration of TCA could lead to better cell-rupture. It should not effect the composition of the metabolome.
I am also not familiar with your assay, but it seems as if it is pH depent, so higher ratios of TCA in your sample lead to a lower pH and could affect the outcomes.
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