Vijithkumar Vijayan

6 Questions 3 Answers 0 Followers

Questions related from Vijithkumar Vijayan

How are the TELL-Seq Linked-Read libraries produced for Illumina short-read sequencing?

Can somebody help me understand the Strand Transfer Complex (STC) formation and the "Tagmenting" process during TELL-Seq Linked-Read library preparation? The link has been provided herewith for...

07 February 2023 8,714 0 View

How to prepare a Rifampicin working solution at concentration of 5μg/5μL, starting with a 450mg drug (tablet) and 100% DMSO?

I am preparing Rifampicin (injectable) with a final required concentration of 5μg/5μL by dissolving a 450mg tablet in 100% DMSO. I want to inject 5μL of the drug solution into my animal model. The...

08 January 2023 4,413 1 View

Determining the centromere sequence of human genome?

Currently, I am working on a project in which I would like to look at the repeats and genes present in the centromere of human chromosomes. Can someone suggest me about a bioinformatic tool that...

28 December 2021 938 1 View

How to retrieve ethnicity specific reference genome (for eg, reference genome for Caucasian)?

I am a bioinformatics student currently performing a human genome analysis. I would like to know how I can download reference genome for caucasian or I would be grateful if somebody can suggest a...

17 December 2021 1,738 3 View

How to test for stem cells?

I manually uprooted two pairs of setae from one single segment of an earthworm - at the cost of bit of tissue being lost from the site of setae, albeit no blood shed - to later observe that wound...

12 February 2021 2,381 0 View

Protein extraction from a non-cellular structure?

I am trying to look at protein in a non-cellular structure using SDS-PAGE. I will be collecting plenty of those structures from the model organism on different days. Is it wise if I collect...

13 January 2020 3,011 6 View