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I want to clone out a particular ALL cell line: RS4;11 after lentiviral transduction (with no puromycin/similar resistance gene unfortunately) to isolate clones with varying expression of my...
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Im hoping to streamline a process which has been established in my lab for some time, but is very time consuming for large numbers of samples. Currently we pellet cells, extract RNA, make cDNA...
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I am trying to create a 16kb construct and transform Stbl3 competent E.coli with the construct. However during restriction digest analysis of my possible constructs, I either see banding patterns...
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Investigating whether it would be possible to do this at least for a few days/weeks. Would appreciate it if anyone has any experiences they would be willing to share. Or even hints and tips for...
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