Victoria J Forster

5 Questions 18 Answers 0 Followers

Questions related from Victoria J Forster

Does anyone have any tips for cloning out leukaemia cell lines?

I want to clone out a particular ALL cell line: RS4;11 after lentiviral transduction (with no puromycin/similar resistance gene unfortunately) to isolate clones with varying expression of my...

12 December 2013 9,102 6 View

Does anyone have experience with analysing amplicon size by Real time PCR?

Im hoping to streamline a process which has been established in my lab for some time, but is very time consuming for large numbers of samples. Currently we pellet cells, extract RNA, make cDNA...

07 July 2013 7,950 2 View

I am using competent E.coli which are EndA+, how do I prevent DNA shearing when preparing my plasmid DNA?

I am trying to create a 16kb construct and transform Stbl3 competent E.coli with the construct. However during restriction digest analysis of my possible constructs, I either see banding patterns...

04 April 2013 284 15 View

Which techniques do you recommend for both intracellular and extracellular ROS detection?

I'm interested in looking at ROS levels in some of my cell lines, both intracellular, and levels in the tissue culture media. I am especially interested in NOx, but a technique detecting many...

04 April 2013 5,016 9 View

Does anyone have any experience of co-culturing HUVECs (or similar) and primary immortalised haematopoietic stem cells?

Investigating whether it would be possible to do this at least for a few days/weeks. Would appreciate it if anyone has any experiences they would be willing to share. Or even hints and tips for...

03 March 2013 8,066 1 View