Valerie Rose Osterberg

3 Questions 9 Answers 0 Followers

Questions related from Valerie Rose Osterberg

Can a plasmid prep be stored in isopropanol over the weekend?

I am doing a Qiagen giga prep on a plasmid that I recently cloned. I didn't realize how long the column would take to drain via gravity, and I have to catch a train. Is it possible to stop the...

04 April 2013 1,998 3 View

Cloning a C-terminal GFP fusion: is it okay that I still have the start codon for both my gene and the GFP?

I have recently cloned some mice which is an expensive endeavor. The construct I was given to clone has our gene of interest tagged to EGFP on the c-terminal end. My job was to put this sequence...

02 February 2013 7,502 21 View

Does the southern blot with biotin-labeled PCR probe detect all gDNA?

I'm trying to use the Li-Cor detection system for a southern blot. To make my probe, I amplify a GFP specific PCR product with biotin-16-dUTP integrated into it. Once hybridized the probe is...

01 January 2013 7,228 7 View