Usman Akhtar

2 Questions 5 Answers 0 Followers

Questions related from Usman Akhtar

How to measure concentration of more than half single stranded olgio and 1/4th double stranded?

I want to anneal two oligos . One oligo is 96 base pair and other oligo is 17 base pair. 17 base pair oligo is complete complementary to 17 base pairs of first oligo after annealing I can measure...

12 December 2017 4,239 0 View

Which site is best for miRNA analysis in vector?

Dear All. I want to clone my target site of miRNA in vector pSEAP 2. So which site is best for insertion? 5' where there are MCS or 3' UTR region for functional analysis? because miRNA binds to...

07 July 2016 7,648 2 View