Uma Nudurupati

6 Questions 16 Answers 0 Followers

Questions related from Uma Nudurupati

Need help trouble shooting veeco 3100 Dimension AFM : Cantilever won't tune ?

I am trying to tune the cantilever HQ:NSC19/NO AL ( https://www.spmtips.com/afm-tip-hq-nsc19-no-al ) on the 3100 SPM Dimension. The manufacturer's suggested resonance frequency is 65 kHz (25-120...

14 November 2019 8,121 1 View

BCA Assay trouble shooting: why is analyate's estimated conc 40% of expected value?

I have been trying to estimate the concentration of collagen type II (tripeptide) in 0.012 M HCl using BCA Assay. I denature the aliquot of protein sample I use by heating it to 60 degrees...

25 March 2019 6,860 9 View

How to confirm contact with the material in rheology? What is the role of normal force?

I have a hydrogel of thickness ~ 4000 um and I want to perform a frequency sweep on it. How do I know that the plate is in contact with the material apart from visually seeing it in contact? I...

23 January 2019 8,352 2 View

How do you choose the wavelength at which turbidity has to be measured?

I have been looking into turbidity measurements in different contexts, and I find that turbidity is measured at various wavelengths depending on the experiment. For example, for bacterial growth...

19 December 2018 7,884 2 View

How to remove MEHQ from Methacrylic acid monomer without reduced pressure distillation?

I am aware that the standard way to remove MEHQ from Methacrylic Acid is to perform reduced pressure distillation. The papers I see suggest using ~ 10 Torr pressure to perform distillation....

23 October 2018 9,803 12 View

TA instruments Rheometer AR 2000: How to change geometry gap for a parallel plate?

I am trying to perform rheology experiments on a cross-linked polymer gel to measure its shear modulus (G) and its G' G" response to an oscillatory stress on an oscillatory rheometer. My polymer...

01 June 2018 1,251 5 View