Tyler T Cooper

4 Questions 3 Answers 0 Followers

Questions related from Tyler T Cooper

Other than HPLC, is there a good method to measure intracellular retinoic acid (RA) concentrations in the cytosol of cultured cells?

I have come across an ELISA to measure RA levels in serum/plasma, however I am not sure if this sensitive enough to determine RA concentrations in cell lysates (0.625-10 ng/mL). I was hoping...

11 October 2017 2,767 2 View

How to get EdU to work with 2nd Ab during IF microscopy?

We have recently encountered an issue where staining for EdU on tissue sections will show EdU+. However, if this is followed by a second Ab against a protein epitope (i.e. insulin) only EdU will...

27 April 2017 6,923 1 View

How to use Flow Cytometry to show Megakaryocyte Endoreduplication?

I have identified CD41+ CD42+ CD45+ human cells in culture, but would like to confirm these are truly megakaryocytes. I was hoping that I could fix the cells and use 7AAD as as a dye to show...

24 January 2017 638 0 View

Is there a way to use immunohistochemistry to show changes in metabolism of murine muscle tissue following schema (glucose vs lipids etc)?

I am using the hind limb ischemia model in NOD/SCID mice and would like to measure changes in metabolism to the localised tissue both in a control mouse and mouse with recovered hind limb...

03 July 2014 7,171 1 View