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Questions related from Tushar Dutta
Has anybody used One Shot® OmniMAX™ 2 T1R Chemically Competent E. coli from Invitrogen? I could not get the result of my cloning with those, while same plasmid was successfully cloned in NEB 5...
06 June 2014 1,231 5 View
I have to quantify the fluorescence of an image by comparing the pixels of fluorescent and normal image which can be done by ImageJ software which is freely available. However I could not do the...
05 May 2014 7,206 1 View
Can anyone suggest a suitable vector mapping software? Suppose I have a sequence of a vector, I want to know the promoter, terminator, antibiotic resistance etc. in a region. I used NEB cutter,...
01 January 2013 7,901 20 View
Is anybody using one shot Omnimax 2-T1 chemically competent E. coli to transform BP reaction products marketed by invitrogen? I have tried those cells and it seems that it's not working at all....
01 January 2013 5,276 4 View
is anybody working on gibson assembly commercialized by NEB? NEBuilder software also helps in designing primer etc. My question is, software predicted annealing temperature differs significantly...
01 January 2013 4,821 4 View
Suppose i have a DNA sequence and i want to find transcription strat site, CDS, poly A signal etc., which software will be useful to find this out?
12 December 2012 6,205 8 View
For purifying attB-PCR product invitrogen suggests to mix 50µl PCR product+150µl TE buffer+ 100µl 30%PEG8000/30mM MgCl2 solution(provided by invitrogen), vortex & centrifuge at 10000g at RT....
11 November 2012 8,443 2 View
I am working on RNAi of nematode genes for which I am adopting gateway cloning strategies. In this strategy to clone the gene in the entry vector (I am using pDONR221 from invitrogen) some adapter...
11 November 2012 3,107 7 View
