Trung Nghia

7 Questions 11 Answers 0 Followers

Questions related from Trung Nghia

Why can't all protein bands be transferred onto the membrane in Western blot?

Hi, I'm stuck with membrane transferring in Western blot. To analyze the expression level of caspase 3 (cleaved, ~17 kDa), I harvested the cell then lysed by Laemmli sample buffer (6X, w/ DTT)...

27 July 2021 8,347 9 View

What happen if the plant extract didn't dissolve well in PBS when apply to the cells?

Hi, I'm checking the cytotoxicity of a plant extract on cancer cells. I prepared my extract in DMSO 100% and make sure it dissolved completely (just by watching it under the light). Then I diluted...

21 April 2021 2,290 3 View

How to dissolve biotin completely when preparing PTM1 Trace for Pichia?

Dear, I am preparing the PTM1 Trace elements for Pichia pastoris. I've known that biotin dissolves weakly in water, especially in low pH. In the PTM1 recipe, the biotin is 200 mg/L. This amount is...

30 March 2018 235 4 View

What is the terminology of the chromatogarphy column made by yourself/myself?

Dear, Please tell me the terminology of the chromatography column which is made manually. I mean, I have to purify of protein in larger scale. I will make a chromatography column by myself, but I...

08 August 2017 7,264 7 View

Why every time the target protein get out of the gel filtration column, it made overpressure?

Dear, I am doing in gel filtration. My protein is fgf2. In the first step of purification, I applied cation exchange chromatography, but it was not clear enough. I try to do the next step to get...

03 July 2017 1,753 5 View

How can I fix unknown lines in SDS-PAGE electrophoresis?

Dear, I take a SDS-PAGE electrophoresis gel. It appears some lines which I don't know what they are. The line connect all wells of gel and they are more than two. What's it and How to fix my...

16 December 2014 6,320 15 View

Why do the compounds from methanol elution in silica gel chromatography not dissolve completely after evaporation?

Hi, I performed a silica gel column chromatography. At the final step, I used 100% methanol to elute all the rest. After evaporation, the dried layer on the glass flask can not be complete...

01 January 1970 5,009 5 View