Trent Place

3 Questions 2 Answers 0 Followers

Questions related from Trent Place

F-Actin immunoprecipitation (IP).

I want do an IP to pull down F-Actin and associated proteins. I have the AbCam monoclonal C4 actin antibody. Do I have any chance of pulling down F-actin or will I predominantly get G-actin?...

02 February 2013 2,476 1 View

Can retroviral transgenes be transcribed/translated prior to integration?

I've noticed that after transducing my pQCXIP-geneX retrovirus into my cells, I have detectable expression of geneX (by western blot) the following day. Am I just seeing the effects of high...

11 November 2012 6,858 1 View

DNA quantification by fluorescent microscopy

I would like to quantify nuclear DNA content in cultured cells for the purpose of identifying copy number changes over time. I don’t need to know specifics of which chromosomes are duplicated or...

01 January 1970 2,218 3 View