Tracy Chia

3 Questions 3 Answers 0 Followers

Questions related from Tracy Chia

Why my Rotavirus Positive Sample Unable to View the gDNA Band on Agarose Gel?

I am extracting DNA from stool samples of rotavirus (RV) )-positive patients. I have observed that all the RV-negative patients can view their gDNA band on the agarose gel while the ones diagnosed...

21 September 2024 9,802 0 View

How much volume of ladder and extracted sample should I add into the well for gel electrophoresis?

If I am planning to mix - Extracted sample: 5μL - Loading dye: 1μL Before transfer it to the well of the agarose gel Is it I need to add 6μL for my DNA ladder? Also do I need to mix loading dye...

22 July 2024 9,096 3 View

How much 1X TAE buffer should I use in preparing 1.2% agarose gel?

Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...

13 July 2024 2,179 5 View