Why my Rotavirus Positive Sample Unable to View the gDNA Band on Agarose Gel?

21 September 2024 0 10K Report

I am extracting DNA from stool samples of rotavirus (RV) )-positive patients. I have observed that all the RV-negative patients can view their gDNA band on the agarose gel while the ones diagnosed with RV-positive can hardly view the gDNA band.

I am running the 1.2% agarose gel with 50 volts for 72 minutes. I am using SPINeasy™ DNA Pro Kit for Feces(MP Biomedicals, LLCQ BioGene) to extract the DNA from the stool samples. I did not use the original stools sample, instead I use the stool samples that has diluted with PBS as the starting material for both RV-positive and negative samples.

I have tried to troubleshoot the lysing steps. Yet, I get the same results. Anyone has the same experience?

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