Toby Samuels

5 Questions 6 Answers 0 Followers

Questions related from Toby Samuels

Why have my cells not evenly dispersed on a haemocytometer slide?

Attempted use of a haemocytometer to count centric diatom cells (~40-50 microns diameter) resulted in diatoms becoming "stuck" at the point where the sample was loaded onto the slide. Therefore...

10 August 2018 9,313 2 View

Lucy 2.0 Question - How do I cut low-quality ends off Sanger reads?

I have tried playing with the settings, changing values for Error (Average, ends) and bracket window - as the software suggests - and I can't get the settings right to remove strings of bases (say...

07 May 2018 8,413 0 View

How do you include filaments in microscopy cell counts?

I have images of polished mineral surfaces that have been colonized by numerous types (shapes, sizes, structures) of microbes. I am have used fluorescence microscopy to image these colonized...

19 September 2016 3,698 4 View

Does anyone have any suggestions for geomicrobiology plate/liquid assays?

I am looking for different plate (or liquid) assays that can be used to identify particular metabolic capabilities of functions of a geomicrobiological community. Any suggestions to add to...

28 July 2015 5,291 2 View

Any advice on the heat shock of Cyanobacteria - temperature switch at 44-46 oC?

In some of my experiments I have been working on heat shock experiments of a temperature range of 40-50oC for 2-30 minute time periods. What I have found is that there seems to be a temperature...

27 July 2015 6,061 2 View