Ting Chung Chau

10 Questions 8 Answers 0 Followers

Questions related from Ting Chung Chau

Can truncated enzyme (in terms of amino acid) be functional, even the active site is remained intact?

Dear all, I would like to ask enzyme remain functional, if part of the amino acid is eliminated and the active site remain intact? Thank you

18 April 2022 9,699 3 View

How can I electroporate plasmid to Lacto species?

Dear all professors, scholars and Drs., I am now facing a problem when I electroporate 15000 bp plasmid to lactobacillus casei atcc 393. I have tried several methods which involved: Methods that...

20 January 2022 5,523 2 View

How to increase the efficiency of apa1 and xbal 1 double digestion of 15000 bp vector?

Dear All, I am now using apa1 and xbal 1 for double digestion of 15000 bp vector. The expected cut fragment size is 2000 bp. However, the cut band is faint which indicates inefficient digestion...

30 September 2021 2,568 3 View

Is there any possibility that 15000 bp vector be cloned successfully into DH5 alpha after ligation?

Dear all, I am now facing a problem. After ligation of a 12000 bp vector with 2000 bp gene fragment, no clones can be obtained after performing DH5alpha transformation? Therefore, are there any...

04 August 2021 1,175 4 View

How to see the expression of 6kda protein?

Dear all, I am now investigating the expression of small size protein (6 kda). However, the expression of the protein cannot be shown clearly after performing western blot. Therefore, are there...

14 January 2021 3,457 3 View

Do I need to add a start codon and stop codon for the sgRNA expression in crispr?

Dear All, I am now designing the sgRNA for knocking out the gene through crispr. Base on my learning experience, a start codon and a stop codon should be put in the N terminus and the C terminus...

17 December 2020 6,053 3 View

Crispr nickase, how to design the homology arm?

Dear All, I am new in the field of the crispr. As far as I know, nickase crispr need two sgRNA. However, I dont know how to design the homology arm in the nickase crispr case. Can u guys give me...

14 December 2020 2,413 0 View

Does anybody know how to lysis the gram positive bacteria with a thick cell wall for doing sequencing?

Any protocol that I follow and what reagents that I should use?

27 May 2020 4,365 5 View

Colony PCR, why my colony PCR result is negative, but the colony can grow in 100 ug/ml amp LB medium?

Hi all, I have picked the colony with pipette and tip the colony directly with the PCR mix. The forward primer and reverse primer that I checked is ok with the melting temp 60 C. Then I...

22 January 2020 6,240 9 View

Urgent! Please help. Problem about cloning and mini-prep. Why I get low concentration of DNA after mini-prep?

In the last few weeks, I have successfully clone a plasmid that consists of GFP and pUC19. Both shows clone on the plates and no clone in negative control plate. After overnight culture with...

18 December 2019 2,504 10 View