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We have found that deleting a transcription factor in Tregs causes fatal autoimmune disorder in mice, concomitant with accumulation of effector memory CD4 cells (CD44hi CD62L low) in blood, LN and...
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Hi we are examining CD81 expression in B cells, macrophages and neutrophils in PBL from mice. To this end, we collected 10 ul blood, lysed RBC, and stained in 10 ul antibody cocktail (B220, CD81,...
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Hi, I have a construct that expresses a certain gene (1.7 kb) together with the downstream GFP; the co-expression is mediated by P2A. In transfection assays, GFP expression was very bright....
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Tet-controlled binary transcription regulatory system is widely used in mammalian cells. Does anyone know if it is applicable to C elegans (in vivo and in cell lines)? If not, why? Thanks Tian
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I am trying to isolate RNA and DNA simultaneously from frozen pellet of mouse sperm. Any advice how to proceed? Thanks!
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