8 Questions 7 Answers 0 Followers
Questions related from Thomas Heida
Having not so much experience with cell-free expression systems, I do have a general question. I am using a commercial RTS 100 E.coli kit to express eGFP. When following the expression on a tecan...
01 January 2019 5,674 3 View
I am trying to prepare hyaluronic acid - PEG hydrogels to which DNA (1200bp) is covalently coupld by copper-free azid click chemistry. Prior to gelation, initial precursor mixture including DNA,...
12 December 2018 7,196 1 View
I am aiming for hyaluronic acid-based microgels particles, which are homogeneously modified with DNA. Therefore, SH-modified DNA, which is additionally functionalized with a fluorescence dye is...
11 November 2018 7,147 5 View
As a chemist, I am working on hyaluronic acid (HA) hydrogels, which are DNA-modified. DNA is attached to HA via Thiol-Michael. However, due to the polyanionic character of HA, DNA forms aggregates...
11 November 2018 5,766 3 View
I am performing cell free protein synthesis of GFP with commercial RTS 5-Prime kit using DNA-modified hyaluronic acid-PEG hydrogel particles. The fluorescently labeled DNA is covalently coupled to...
11 November 2018 9,801 3 View
As a chemist, I am working on hyaluronic acid (HA) hydrogels, which are DNA-modified. DNA is attached to HA via Thiol-Michael. However, due to the polyanionic character of HA, DNA forms aggregate...
10 October 2018 6,672 5 View
Acryloyl chloride disolved in toluene is added dropwise to an ice-cooled NaOH solution (0,4 M) of N , N -bis(carboxymethyl)- L -lysine Thanks for helping me out !
05 May 2016 8,493 5 View
I am using homemade NTA-modified hyaluronic acid particles for deGFP-His purification from E.coli based CFPS mixture. On SDS-PAGE I clearly see removal of smaller proteins (< 20 kDa) but there...
01 January 1970 8,980 4 View
