Thien Ngoc Le

8 Questions 16 Answers 0 Followers

Questions related from Thien Ngoc Le

Can RNAs with Phenol contaminant interfere RT qPCR?

Hi Fellows, I obtained RNAs with low purity (A260/280=1.2-1.8) and phenol contaminant. I reckon the impurities can interfere reverse transcription (RT) PCR for gene expression. How is your...

14 July 2025 5,015 3 View

How many Acute Phase Proteins have been used in diabetic testing? (excluding Albumin and Fibrinogen)?

A list of current protein biomarkers has been shown in our systematic review, including Albumin, Fibrinogen, and eleven Acute phase proteins. Comparisons between healthy and type 2 diabetic...

18 June 2025 5,917 0 View

Which consitent changes in haematological profile of Type 2 diabetic patients can be used as biomarkers?

I found consistent changes in haematological profile of T2D patients, that can be used as clinical biomarkers including: increased count numbers of white blood cells (WBC) and platelets; decreased...

03 June 2025 4,144 1 View

Twenty-four hours are enough for human dTHP-1 macrophage differentiation in vitro?

Hi ALL, I constantly differentiate human macrophage from THP-1 cell line using 100 ng/mL PMA (phorbol 12-myristate 13-acetate) for only 24h (DAY 1), then change the media (RPMI) and leave them...

25 May 2025 3,252 4 View

Is TGF-β used in a blood test to monitor the progression of type 2 diabetes?

TGF-β1 is an important factor in diabetic nephropathy. Are TGF-β1 levels elevated or reduced in the serum of T2DM patients?

13 May 2025 9,020 5 View

Which anti-inflammatory cytokines/biomarkers from human macrophages show downregulated expression in poor-controlled type 2 diabetes?

T2D is quite a complex scenario with up- and down-regulation of cytokine expressions. In case of poor controlled T2D (contribution of hyperglycemia/high blood glucose and glycated serum...

10 May 2025 6,984 4 View

LDL can be used as a biomarker for lipid screening in diabetic patients?

Dear Fellows, Looking at Lipid profile of diabetic patients, there are consistent changes in HDL, TC and triglycerides, which can be used as biomarkers for screening/controlling lipid levels in...

01 January 1970 9,183 10 View

Diabetes Atlas - Top 3 countries with the highest rate of diabetes prevalence in adults

Diabetes Atlas IDF 2021 - Top 3 countries with the highest rate of diabetes prevalence in adults (20–79 years) are Pakistan (30.8%), French Polynesia (25.2%) and Kuwait (24.9%). The most...

01 January 1970 9,232 7 View