Tenzin Adon

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Questions related from Tenzin Adon

"Does unstained control MCF-7 cell produce a peak in cell cycle analysis?

I have given the MCF-7 treated cells and control for cell cycle analysis by FACs. Unstained control MCf-7 cell have produce a peak in cell cycle analysis. So this result is wrong? if it is wrong,...

07 January 2025 4,487 4 View

Can lower test compound concentration (10 uM) can exhibits higher % growth inhibition of cancer cell than higher concentration ( 100 uM)?

I am inexperienced in conducting in vitro studies, an area outside my area of expertise. I have been training in basic cell culture technique for the past 2 months. Currently, I am evaluating the...

01 January 1970 9,085 5 View

"What explains the difference in results between cell cycle analysis (flow cytometry) and apoptosis assay (AO/EtBr staining)?"

"Is it possible that a test compound (Compound A) showing a higher percentage of sub-G1 cells by flow cytometry could still show a lower percentage of apoptotic cells compared to another test...

01 January 1970 4,613 7 View

Distorted cell cycle profile due to high % apoptosis.

Are distorted cell cycle profile of cells treated at IC50 values for 24 hours due to high apoptosis (22% in one case and 43% in another case of test chemicals) acceptable? Can't I interpret the...

01 January 1970 6,977 2 View