Tejas Karhadkar

5 Questions 4 Answers 0 Followers

Questions related from Tejas Karhadkar

Does anybody know how to cut open the C. elegans worm to have its Gonad intact for immunofluorescence staining on a (+) slide?

I have to stain the gonad of the C. elegans (outside the worm). For the same I need it to come out intact without breaking anywhere from the distal to proximal end. Does anybody have suggestions...

10 January 2014 8,112 3 View

How do I isolate/recover the GFP positive embryos from the slide?

I prepare the slide with agarose patch on it and synchronize the embryos of C. elegans on it. I check for the GFP positive embryos from that synchronized group, on a fluorescent microscope. And...

16 August 2013 1,965 1 View

What is the ideal dilution for using the Primary and Secondary Antibody Concentration for 6x HIS tag : Protein?

The 1:2000 for primary and 1:20000 for secondary gives a very dark background .

23 May 2013 4,924 3 View

What if the restriction digest pattern of plasmid midiprep varies from the same plasmid's miniprep?

I normally inoculate 5 - 7 colonies from selective antibiotic LB plate after transformation in the liquid LB with the same concentration of the same antibiotic. The next day, I do plasmid miniprep...

28 February 2013 4,996 12 View

Gateway Cloning - BP Clonase - BP Reaction

I want to create an entry clone and I am using Invitrogen's BP Clonase Kit. As per the manual, the Forward Primer with attB1 sites has two unspecified nucleotides as "NN". Our lab uses "TA" in...

17 February 2013 5,411 1 View