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Questions related from Tejas Karhadkar
I have to stain the gonad of the C. elegans (outside the worm). For the same I need it to come out intact without breaking anywhere from the distal to proximal end. Does anybody have suggestions...
10 January 2014 8,112 3 View
I prepare the slide with agarose patch on it and synchronize the embryos of C. elegans on it. I check for the GFP positive embryos from that synchronized group, on a fluorescent microscope. And...
16 August 2013 1,965 1 View
The 1:2000 for primary and 1:20000 for secondary gives a very dark background .
23 May 2013 4,924 3 View
I normally inoculate 5 - 7 colonies from selective antibiotic LB plate after transformation in the liquid LB with the same concentration of the same antibiotic. The next day, I do plasmid miniprep...
28 February 2013 4,996 12 View
I want to create an entry clone and I am using Invitrogen's BP Clonase Kit. As per the manual, the Forward Primer with attB1 sites has two unspecified nucleotides as "NN". Our lab uses "TA" in...
17 February 2013 5,411 1 View