8 Questions 6 Answers 0 Followers
Questions related from Teadora Tyler
Hello! I want to try Npas1 staining on post mortem FFPE brain tissue, but I have no previous experience with Npas1 and am not sure which antibody to start with. If anybody has good experience with...
26 April 2024 6,707 0 View
Mitochondria evidently have important roles in cancer development and progression. So when preprocessing single cell data generated from tumor tissue, is it wise to apply the "standard" filtering...
21 August 2023 4,591 2 View
What is the current opinion about the Abercrombie-correction and its modifications? Most literature I find on the topic is at least 20 years old.
07 March 2022 1,367 1 View
We tried several, with different dilutions and pretreatments, but no results. Literature is full of rodent experiments, but data on post mortem human tissue is scarce. The paper describing VIP in...
04 December 2020 7,987 0 View
For example, I have a 2-level factor variable (diagnose) in interaction with a 6-level factor variable (layer). Intercept by default is the first level of all. In my case intercept is control...
19 September 2020 6,498 4 View
Does anyone know how this number became "default"? what is it based on? How deep is our knowledge of "average" , "healthy" gene expression of different cell types? Sorry if my question is trivial...
05 August 2020 7,381 1 View
The long process from fresh frozen tissue to PCR-ready cDNA seems to me as something quite uncertain. A LOT depends on how well the microdissection goes; and even if you manage to dissect exactly...
01 January 1970 5,165 7 View
I have seen extensive doublecortin (DCX) mRNA expression in single cell RNAseq data generated from the human striatum. (And some other markers usually associated with neuronal migration, such as...
01 January 1970 6,649 0 View
