Tanmoyita Nayak

9 Questions 20 Answers 0 Followers

Questions related from Tanmoyita Nayak

Potential Challenges of using Cold WFI/water during downstream process?

Hello all, Can anyone share their input on what are some potential challenges if I use cold WFI (2-8 degree) to start flushing ÄKTA equipment, during polishing steps (AEX/CEX) for monoclonal...

01 April 2022 5,423 2 View

Interpretation of Mass-spec data?

Hi, I recently got data from an IP-MS (with chemical labeling) to get an idea about the interactome of my protein of interest, however there is a particular tendency of a certain protein and their...

26 July 2017 631 7 View

Help in interpreting cell cycle data?

Hi, I am doing cell cycle comparison in HEK cells between my control Vs. treated cells. In my treated cells, there is almost no change in G1, 10-12% decrease in S-phase and 5-6% increase in G2M...

07 March 2017 5,749 2 View

Best cell synchronization method after transfection?

Hi, I am working with a slow proliferative cell line (doubling time 18-20 hours) and I want to perform cell cycle analysis by FACS after transient transfection with my protein of interest. I did...

13 February 2017 2,068 2 View

Exact opposite result in cell cycle phase at different time point after overexpression?

Hello, I did a general comparison of cell-cycle analysis between my protein/CNT overexpressed HEK cell poupation. However, after 24hour of transfection, it shows a 10% increase in S-phase whereas...

08 February 2017 2,909 5 View

Are the RNeasy mini spin columns same in RNeasy plus mini kit and miRNAeasy mini kit?

Hallo everyone, I am pretty new in RNA-field and I want to do RNA extraction and RNA-Seq later on. However, I am a bit confused about the difference between the qiagen RNeasy mini spin columns in...

21 March 2016 9,619 5 View

Can someone help me with a low MW protein blotting problem?

Hi all, I am working with an intracellular domain of a type1 transmembrane protein. This intracellular domain is really small (9 Kd) and contains a predicted NLS. I made two constructs (with two...

20 August 2015 2,074 8 View

Recombinant protein expression: DNA sequence of insert is right, still no protein expression after transfection...?

Hello all, I am dealing with a transmembrane protein and trying to create constructs with different domains of it. The one I am particularly interested in is the intracellular domain (8.5 Kd)...

26 June 2015 9,406 4 View

Any suggestions on why tag is not working in western blot?

I made a clone with my insert (230bp) and vector (5kb) which is c-terminally flag tagged. After making the constructs I have checked by sequencing twice! (it`s sure that there is not...

29 April 2015 5,631 16 View