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Questions related from Szymon Gaweł
Hello, I ran into a serious problem during my experiments. For some reasons, I need glycine at the N-terminus of my ovalbumin (mammalian cell expression, slightly modified protein, ordered from...
08 September 2021 1,586 3 View
I have question as described in the title - how can I add residues to protein, with the PyMol Builder, and mark them as non-organized? PyMol gives options to add them as beta-sheets or...
28 February 2021 5,605 2 View
Hello all, I am currently trying to modify my plasmids so that it has a sequence coding the pelB or ompA tag in front of the proteins. The problem is that in order to paste such a sequence into...
26 November 2020 8,524 3 View
Good morning all, My question is just like in title. I was using EDTA when collecting mouse blood, and most of ELISA kits are dedicated for plasma gained from blood collected with citrate or...
16 April 2019 2,869 2 View
Hello all, I have a question about the use of EPR to study membranes in cells. I would like to conduct research on blastocysts and oocytes, but I am afraid that a large number of them will be...
08 April 2019 6,558 0 View
Hello all, my question is about using my frozen samples to detect macrophages. I have uterine cells, scratched with 40um strainer and frozen in -81 degrees in 90% FBS and 10% DMSO medium. They...
01 April 2019 1,937 3 View
Hello, I am student and I need to know one thing. How can I calculate linewidth in EPR spectra, if I know effective relaxation time, g-factor and band frequency? I'll be glad for any help, i...
03 September 2018 7,183 2 View
