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All the protocols to detect beta galactosidase activity in a cultured cell includes washing/fixation and addition of x-gal. Can x-gal be directly added to the cell culture media...
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Hi, I have recently done some qPCR after total RNA extraction, followed by first strand cDNA synthesis. The problem is, while one treatment is giving me closely lying values for relative gene...
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I have a DNA sequence and I suspect that it forms a curved structure, however, while I am planning the experiments to test this hypothesis, I would like to do some in-silico prediction to help me...
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Hi, I want to study the structure of the DNA-protein complex using AFM. The problem is I don't have purified protein with me. So, as an alternative, I am planning to incubate the DNA fragment with...
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Hi, I am doing qPCR assays with S. cerevisiae. As per the article "Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces...
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Hi, I want to know if TritonX-100 can be used instead of 0.1%Nonidet-P40 7 for Co-IP lysis buffer? The proteins I am targeting are both nuclear proteins.
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HI, I am using the TRIzol reagent for RNA extraction from yeast. I am planning to shift to a kit based protocol for better reproducibility and consistency. I looked for many RNA extraction kits...
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Hi, I have to see some protein-protein interaction for which I am using the yeast two hybrid system. After constructing the fusion GBD and GAD constructs (in pGBD C1 and pGAD C1 vectors) I want to...
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