Sushil Kumar Middha

5 Questions 23 Answers 0 Followers

Questions related from Sushil Kumar Middha

Which type of sequencing is best suited for 300bp PCR product?

Hello every one, Can you please suggest me which method should I use for sequencing a 300bp PCR products either Illumina or Oxford Nanopore. I have around 100 PCR products which are about 300bp long.

01 November 2017 6,221 8 View

Can someone help me in compound isolation( from crude extract) purification, identification and structural elucidation?

Is there anyone who can suggest me about isolation and purification of a specific compound. After running a column (solvent Ethyl acetate), I am getting a lot of compound in the same fraction. If...

21 September 2016 4,384 10 View

Can I use Tyr-Kinase as receptor for docking in my breast cancer studies?

I have used EGFR/Tyr-Kinase for my docking studies. But I have got one dough like the inhibitor I use, will also stop other growth regulations. So I would like to know is this fine to go ahead.

30 March 2015 8,502 4 View

What would be the specific dose for my drug in for animals use?

I have got LD50 value for my plant is 5.18 gm/kg BW. Then, what would be the correct dose to use orally for the further study. My research area is diabetes using Wistar rats as the animal model.

21 February 2015 4,758 5 View

Hi eVERYONE

cAN WE DISCUSS REGARDING DIABETES.WHY HERBAL TREATMENTS ARE NOT PERFORMING WELL IN REGARD IF THIS.THERE ARE HUGE NUBER OF PAPERS CAN ANYONE FROM GROUP HELP ME REGARDING THIS.

29 October 2010 2,686 0 View