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Questions related from Sungjin Lee
I am using wash buffer with conductivity of 5.5 mS/cm. However, during wash stage in ion-exchange, lower conductivity (around 4.5 mS/cm) is observed even after the 1 CV. Is there any reason that...
01 July 2020 3,668 0 View
I'm trying to analyze the soy protein hydrolysate using Q-TOF MS. My sample dissolve in 4 M TFA. Is 4 M TFA available for Q-TOF MS?
17 June 2020 5,795 0 View
I'm culturing CHO cell using media of SAFC bioscience. When culturing at 20 ℃ , there is no pellet. However, when culturing at 37 ℃, the brown pellet come out after centrifuge. Is there any...
12 June 2020 3,928 3 View
I found that the matrix of the captureselect IgA affinity resin is consist with VhH domain (nanobody) of the IgA. Is there any available structure of captureselect IgA affinity resin? Or is there...
01 June 2020 4,004 0 View
Is there any concentration range for the CD spectroscopy? I prepared the sample which has 220 uM and its result is not repeatable Is there a possibility that the sample is highly concentrated for...
22 October 2015 4,308 5 View
Hi, I'm using Sartorius Vivaspin 6mL and 20mL (3000MWCO) for concentrateD protein solution. Can the Vivaspin tube reused? Also if I want to reuse that, is there any thing to treat between two usages?
06 November 2014 4,926 11 View
Hi~ I want to prepare the sample which is a complex of DNA and Protein. My DNA is 27bp and my protein is 16kDa. I want the mixture sample for the NMR spectroscopy. If I mix protein and DNA,...
03 November 2014 8,856 10 View