15 Questions 15 Answers 0 Followers
Questions related from Sumit Mukherjee
Hi, I have encountered a weird problem. I have been trying to express a protein fragment from bacteria. This fragment has an N-terminal HA and a C-terminal 6X His tag. Following expression, I...
23 November 2020 2,697 4 View
Hi,
14 September 2020 9,855 2 View
Hi, I am planning to do Acyl Biotin exchange assay on red blood cell ghosts to detect the palmitoylome of the RBCs. Also, I want to treat the RBC ghosts with 2-BP and palimotoylation inhibitor and...
03 July 2020 1,894 0 View
Hi, I am working with a protein that is typically secreted from the parasitic cells that I study. I am trying to express and purify it from HEK cells. Despite of using the mammalian signal...
02 April 2019 7,350 5 View
Hi, My cells express a HA-tagged (C-terminal) protein that I am trying to pull down using the anti-HA antibody coated dynabeads. Interestingly, I see degradation of protein in the pull down...
24 February 2019 1,218 6 View
Hi, I am working on a protein for which we don't have an antibody. This is a protease and we are interested in determining it's functionality. We tagged the protein at the C-terminal end with HA...
15 February 2019 4,295 9 View
Hi, I have a very general question. Since PBS is a isotonic solution, I was wondering if 2X or 0.5X PBS could be considered as hyper or hypo osmotic conditions respectively? I know that typically...
23 September 2017 7,435 3 View
Hi, I was wondering if any one could explain me the relationship between mitochondrial membrane potential and superoxide production? In order to maintain its functionality, mitochondria need to...
15 September 2017 8,140 3 View
Hi, I am studying ROS accumulation in the mitochondria of Leishmania parasites, that are defective in sterol biosynthesis. Staining with MitoSox shows that the mutants accumulate very high level...
19 February 2017 6,255 1 View
Hi, I study lipid metabolism in Leishmania parasites. For this I have generated mutant parasites that are defective in sterol biosynthesis. Recently while characterizing these mutant parasites, I...
15 February 2017 7,659 2 View
Hi I study lipid metabolism in protozoan parasites. I have created mutant parasites tbat are deficient of genes involved in sterol biosynthesis. I wanted to analyze the physiological alteration...
18 January 2017 7,919 3 View
Hi, Attached is a sample picture of Leishmania parasites that are stained with mitotracker. This dye gets accumulated into the mitochondria in a potential dependent manner. So, accumulation of the...
12 December 2016 5,466 3 View
Hi, I would like to carry out an in vivo mice infection experiment with Leishmania parasites where I want to knock down my gene of interest in once the parasite is in the mice. Initially I would...
17 November 2015 7,148 7 View
Hello, I have been trying to knock down a particular protein in Jurkat cells using the Clontech's pSingle shRNA vector system. This is a doxycyclin inducible system. Initially I followed...
16 September 2014 6,809 5 View
Hi, I would like to transfect Jurkat T cells with some plasmids. So far I have used commercial reagents and every time got to see transfection efficiency nearly 70-80%. However, since these...
06 August 2014 3,795 1 View
