20 Questions 23 Answers 0 Followers
Questions related from Sumanth Kumar
Hi, I have been awarded the DBT-CTEP travel grant to attend the conference "64th Annual Meeting of the Biophysical Society" in San Diego which is going to be held between 15th to 19th February,...
23 January 2020 6,596 1 View
It is well known that bonding between Streptavidin (STV) and biotin is very strong. As a part of my experiment I would like to check if STV binds to biotin which is attached to PEG. The surface...
20 July 2018 1,597 3 View
I am trying to measure stiffness of red blood cells in RPMI using AFM force spectroscopy method. In literature, the typical indentation of RBC at a force of 500pN is 500nm. But in my case I see...
11 July 2018 7,913 5 View
I would like to perform force spectroscopy on RBC using AFM. For this purpose I need 50ul of blood which would be diluted further in RPMI or PBS at pH7.4. In literature I see that disodium or...
27 June 2018 6,173 6 View
I am performing AFM force spectroscopy on red blood cells by immobilizing them on glass surface. I clean the glass coverslips by sonication in soap solution, Milli Q, ethanol, acetone, ethanol for...
26 March 2018 763 3 View
I would like to prepared a DNA sample that has -SH or thiol group at one of its end so that I can covalently immobilize it on a surface. The -SH modified primers are commercially available. I am...
20 December 2017 5,700 6 View
I am using triangular MLCT probes purchased from Bruker. I would like to measure the spring constant of cantilever using thermal noise method. I have measured time series of cantilever deflection...
15 November 2017 7,542 5 View
I would like to attach DNA to AFM tip and image it using SEM to check if coating was done properly. I couldn't find any papers that show SEM images of DNA. I would like to know the protocol to do...
13 November 2017 4,328 2 View
I have purchased human histone octamers from Sigma (SRP0408) for nucleosome reconstitution experiments. I would like to check the protein complex in octameric form. Running it on SDS-PAGE will...
31 October 2017 3,403 5 View
I am trying to measure the bond strength between streptavidin and biotin which was reported in literature to be around 150-250pN. In my case I have NHS-PEG-Biotin on mica sheet and streptavidin on...
29 October 2017 3,797 3 View
I am using syringe pump with the flowrate set to 5ul/min. When I flow water which is in plastic syringe through a 1mm 0.5mm tube and collect the liquid for 10min. Then I see that ~50ul liquid is...
26 July 2017 2,696 2 View
I would like to prepare mono nucleosomes for my single molecule experiments. We have PGEM 3Z vector (size is 3kb) from which we are amplifying the 344bp oligo that contains the Widom 601 sequence...
15 July 2017 9,089 1 View
I am trying to perform APTES coating on glass coverslips and AFM tips. I need the most anhydrous organic solvent to dissolve APTES so that there wont be any polymerization of APTES. I have seen...
04 July 2017 4,647 3 View
I am trying to measure the affinity of biotin to bind to streptavidin using AFM force spectroscopy. I would like to know the what buffer should be used and the pH to be maintained in which the...
30 June 2017 7,771 3 View
I would like to coat AFM tip with APTES and found that 10% APTES is the solution in which tips are placed and incubated for 2hrs. I have seen papers mentioning the usage of solvents like acetone,...
07 June 2017 8,016 15 View
At some points in space the director of the molecules in nematic crystals is undefined which are called defects. Disclination line is one of the types. I find it difficult to understand what it...
30 December 2014 6,817 1 View
In a birefringent crystal, the incident light gets split into two beams. One is ordinary and the other is extraordinary rays. The ordinary ray experiences constant refractive index n_o but the...
14 October 2014 8,159 9 View
In SPDC type-II, there are two intersecting cones emerging from the birefringent crystal. Each cone has photons of either horizontal or vertical polarization. But the polarization of photons is...
08 October 2014 7,376 7 View
Please provide me a reference to find the calculation for this.
07 October 2014 1,523 6 View
I read that polarization is directly proportional to electric field in a linear medium. Here we neglect the higher order terms in the Taylor expansion of polarization as the values that non-linear...
07 October 2014 9,529 5 View
