Sukanta Bhattacharya

4 Questions 11 Answers 0 Followers

Questions related from Sukanta Bhattacharya

Can anyone share the experience in reversing the column in HPLC?

Of late I am experiencing very high backpressure during my HPLC runs which I think may be due to some blockage/precipitation in the column. To resolve this issue, I am considering reversing the...

09 September 2015 8,343 11 View

Can somebody suggest how to get crisp bands on PAGE after qPCR of a 60 bp oligo using Brilliant III mastermix in ABI 7800(old) system?

I am using Brilliant III qPCR mastermix to amplify a 60 bp oligo and getting spurious bands on 12% PAGE when I try to amplify using 95 degree denaturation for 15 s followed by 60s extension for...

05 May 2015 6,856 3 View

Is there a rapid method for simultaneous detection and quantification of HMW and LMW Hyaluronic Acid?

A HPLC based method would be highly desirable, but any other technique that may do the same would probably be okay.

07 July 2013 931 3 View

Can somebody suggest how to perform Genetic Algorithm in 2012 version of Matlab?

I can find the optimization interface but m unable to find GA.

09 September 2012 4,587 2 View