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Questions related from Sujit Pujhari
Hello, I am referring the book chapter "Application of CRISPR/Cas9 to Autophagy Research" https://doi.org/10.1016/bs.mie.2016.09.076 to generate a Huh7, ATG5 KO cell line by NHEJ. I am getting...
05 April 2018 2,520 1 View
I am working on a gene expression experiment using SYBR green RT-PCR. I see perfect single peak melting curves for reference gene in my control samples but curves are erratic for treatment sample...
06 March 2016 1,769 3 View
I received a plasmid from a research group and suppose to make RNA from it for transfection. The plasmid has a T7 promoter, I also noted that it needs "A cap" instead of the more common "G Cap" at...
16 December 2015 8,969 2 View
I am using iq5 bio rad real time pcr machine. I want to use the std curve from my previous experiment to analyse my data.
20 April 2015 7,381 2 View
I am working on a co-localization experiment. I have shown that my protein of interest interacts with ATP5A, an inner mitochondrial membrane protein, by co-IP experiment. Now I want to do...
28 January 2015 3,234 4 View
I was going through a couple of articles related to virus induced IP3K/Akt pathways. In some of those, I found people have serum starved the cells before virus infection or IP3K inhibitor...
12 June 2014 6,665 2 View
I want to pull down a membrane bound cytoplasmic GFP tagged protein. For this I ordered GFP tagged beads from chromotech. I am lysing the cells expressing GFP tagged protein of my interest with...
16 September 2012 4,102 4 View
I am doing an apoptosis assay using dual staining with PA and Hoechst. Regarding the calculation percent of apoptotic cells I am little bit confused; Is this procedure right? I have 3 types of...
19 April 2012 4,856 3 View
