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Questions related from Sue yu
According to the ChIP-seq results, 90% binding regions of protein A are located in intergenic. So what could be the function or consequence by binding to intergenic regions? Is there any example...
07 July 2017 7,645 6 View
Mouse embryonic stem cell medium usually contains supplementation of MEM nonessential amino acid. I am wondering whether MEM nonessential amino acid essential for culturing mouse ES cells. Is...
04 July 2017 7,851 5 View
The protein X I am studying may related to RNA binding, so I am interested to test the RNA that can be bind by protein X. I read some paper, and found that there are several kind of methods and...
20 April 2016 6,609 3 View
The protein I am studying has a domain may related to RNA-binding, but so far no paper showed it could bind to RNA. I want to test whether it can bind to RNA directly, what kind of methods can be...
04 November 2015 2,516 12 View
I want to test whether two proteins A and B could interact with each other, and I did Co-IP. According to my result, I found that B-IP could detect A, but I can not detect protein B in anti-A-IP....
30 August 2015 6,079 4 View
Anyone knows the answers for this question, many thanks.
17 July 2015 5,206 7 View
I want to amplify 300bp genomic sequence using PCR, what is the template for PCR? cDNA? genomic DNA or chromatin? Thanks.
14 May 2015 6,545 2 View
According to my previous progress, I suppose one protein X is involved in elongation, so my idea is to test whether there is elongation defect upon knockdown protein X. but I don not know what...
14 May 2015 3,826 2 View
