Sudhansu Sekhar Choudhury

5 Questions 13 Answers 0 Followers

Questions related from Sudhansu Sekhar Choudhury

What are the best and possible methods fro separation of glucose molecules ( monomere & dimer) from a polysaccharide mixture?

Her, I have a Dextran ( mw : 15, 000) , a bioplymer polysaccahride, and i m perfoming the hydrolsysis part by acid treatment & higher temp. to get desired molecular weight of polysaccaride (...

08 August 2019 4,496 4 View

Can anybody suggest me how to do E-rosette inhibitionand lymphocytotoxicity assay in antithymocyte immunoglobulin (ATG) in a descriptive manner?

Hello I purified a specific antibodies (polyclonal) named as antithymocyte immunoglobulin (ATG) from immunized horse plasma by affinity chromatography, now i need to do certain potency test in the...

06 June 2018 7,610 0 View

How to reduced the osmolarity of amino acid mixture (16 Amino acids) having osmolarity 1020 mOsmol to 700 mOsmol?

I prepared a amino acid mixture (16 amino acids) by using HCl, but the osmolarity of the mixture is coming to 1050 mOsmol, i need to maintain the osmolarity in the range of 600 to 700 mosmol,...

12 December 2017 3,339 2 View

How much quantities of thymocytes (antigen) and how many days need to inject to horse for developing antibodies (antihuman thymocytes)?

I need to rasise antibodies against human thymocytes (ANTI HUMAN THYMOCYTES) in horse by injecting human thymocytes as a antigen, any body can suggest me , how much quantities of thymocytes...

03 March 2017 3,182 2 View

What is the best and standard protocol for isolation and purification of thymocytes from human thymus?

Dear colleaguge At present i m working on certain Immunology based experiments, and i m planning to isolate and purify the thymoctes from human thymus which i will use further as a antigen for...

02 February 2017 5,003 5 View