I prepared a amino acid mixture (16 amino acids) by using HCl, but the osmolarity of the mixture is coming to 1050 mOsmol, i need to maintain the osmolarity in the range of 600 to 700 mosmol, anybody can suggest me how to reduced the osmolarity values in my amino acid mixture, what method or what type of chemicals I need to use for reducing the osmolarity?
As indicated the number of particles (molecules for not dissociating compounds and ions) contribute to the total osmolarity of the solution. In my opinion you may try to preparare a solution without using HCl. This is a strong acid, completely dissociated and contribute to the osmolarity of the solution with a coefficient of 2 in respect to its concentration since one mole generate two ions (so two particles osmotically active).
Dear Venditti, than q so much for sharing ur openion , actually i m solubilizing all 16 amino acids by using HCl, until unless its very difficulty to solubilize all the amino acids with out HCl, i agree that beacuse of the presence of HCl only its getting higher osmolarity, or else if u have any idea how to solubilize all 16 a.a except HCl, pls share ur openion, where i cannot get high osmolarity
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