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Questions related from Subrahmanya Hegde
I am working on a weed called Parthenium hysterophorus, I am facing a problem where I am not able to get a proper reproducible germination in lab conditions, and also I am facing difficulty in...
20 April 2024 7,520 2 View
I am facing a germination problem in the seeds of some weed species, which I am working on, because of seed dormancy. to break the seed dormancy I need to provide gibberellic acid in the MS media,...
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I am growing Arabidopsis (Col-0) in both +P and -P medium (JOHNSON'S medium) the plants which are growing in -P showing the anthocyanin accumulation in their leaves when it compared to plants...
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I have captured the chromosome set of some plant species, and now I want to measure them and arrange them based on their size, so can anybody please suggest a good and efficient karyotyping tool...
08 October 2023 9,876 1 View
I am getting the desired amplification with normal DNA Taq polymerase but I am not getting it with high-fidelity Taq polymerase, I am getting non-specific amplification. so can I go for sequencing...
06 September 2023 3,237 3 View
What may be the possible reasons for getting non-specific DNA amplification which is amplified by high-fidelity taq polymerase? Even though we get good desired amplification by normal Taq polymerase.
06 September 2023 2,076 3 View
We have isolated rice plant DNA by the CTAB method, and we are aiming to amplify the particular region of rRNA. We have standardised the universal primers as well, which are working fine for other...
13 June 2023 2,865 1 View
