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Questions related from Steven Arcidiacono
10-fold dilutions of phage (initial concentration 3E9 PFU/ml) + bacteria (1E5 CFU/ml) were run in triplicate on microplate. Plate was incubated overnite at 37oC, with OD600 measured every 30 min....
09 June 2022 3,480 3 View
Hello, I've blotted a 30kDa protein to nitrocellulose from 12% Bis-Tris NuPAGE gels run in MES or MOPS buffers, and transfer from MOPS seems less efficient from MOPS gels. I understand that MOPS...
19 February 2020 7,158 0 View
Hello, Can anyone suggest a non-sequencing alternative to DGGE analysis for fecal samples? DGGE is very time consuming for quick analysis and seems to be more "art" than science. If no good...
22 July 2019 1,126 0 View
Is there a way to in "real time" monitor bacterial growth of in culture media with insoluble or semi-soluble components (think fiber, mucin, etc)? Looking for a method to replace track optical...
15 January 2019 2,052 0 View
