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Questions related from Stephen Lippi
We are looking at collecting blood to assess resting glucose levels. However, with several animals going through terminal procedures, what is the best way to store collected blood? (Ice, dry ice);...
12 April 2019 6,629 3 View
How many antibodies (of different molecular weights to distinguish from one another) is it ok to run on a particular gel during western blotting? I ran GFAP, GAPDH, and BDNF all on one blot as...
27 February 2018 5,832 2 View
Can someone please explain why it's so common in the literature using the Barnes Maze to have a 15-minute intertrial interval? Protocols for other methods of measuring spatial memory have...
17 January 2017 7,890 1 View
In breeding two different strains of transgenic mice, I had a small yield of "true" wildtype animals (negative on all of the genes/promoters of interest). However, I did get a good amount of...
07 December 2016 8,995 3 View
We are currently breeding J20 males and rTg4510 females from the Jax Lab and are having breeding difficulties. We pair for 2 weeks (we also give Lovemash throughout the breeding period). Has...
19 September 2016 9,568 3 View
My question concerns exposure times and fluorescence values. I am imaging brain slices under an Olympus BX50 fluorescence microscope with a FITC cube & mercury burner. I have brain slices that...
24 September 2015 9,655 9 View
I'm preparing to run Western blot analyses on rat tissue and have read some estimates where ~5mg of tissue is used with 300uL of lysis buffer. However, a hemisphere of a 4-month old rat weighs...
14 July 2015 3,692 5 View
