Stephanie Morphet

4 Questions 4 Answers 0 Followers

Questions related from Stephanie Morphet

Will adding restriction sites to my construct affect peptide folding?

Hello RG! I've designed a construct that will be expressed off a plasmid. I would like to add some restriction sites so I can swap out parts of the construct later on. My concern is adding...

20 May 2019 2,708 3 View

Most hydrophobic 96 well plate available?

Hi everyone! Does anyone know what the most hydrophobic plate on the market is? Is there a plate more hydrophobic than the polysorp plate? Also, does anyone know how the polysorp and...

18 January 2017 8,049 4 View

Do I need to work aseptically with phage display to prevent contamination in my e. coli cultures when I amplify my phage?

Hello everyone! I'm starting a project involving phage display and the protocols largely sound like I can do my panning on the bench top, instead of in a biosafety cabinet. Do I need to pan...

12 January 2017 2,680 1 View

Can I stain a TLC plate multiple times, using different stains?

I'm trying to identify some spots on my TLCs. I'd like to stain first with alpha-napthol and H2SO4, and then stain with Dittmer reagent or Dragendorff reagent. Can I do that?

02 March 2015 5,902 3 View