Stefanie Becker

2 Questions 3 Answers 0 Followers

Questions related from Stefanie Becker

Is there any reason why I can not order an oligo and use it directly as an insert for SLiCE cloning?

I was about to design the primers for SLiCE, when I realized the insert is so small I could order it as one oligo and it would not even be that expansive. As its quite some time ago, that I used...

19 March 2020 3,138 3 View

How do I prevent protein recipitation in the first step of protein extraction for proteomics?

I plan to follow a protocol that we use in our lab for protein extraction, however I am afraid to lose proteins in the first steps. We start with frozen cell pellets of gram negative bacteria...

11 February 2019 7,070 6 View