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Questions related from Stefanie Becker
Dear Community, I have predicted the structure of a transmembrane protein with 5 transmembrane helices. When I use Modeller it is not confident with the last helix, so I end up with a structure...
31 March 2025 549 3 View
When culturing bacteria and comparing two conditions—one with a specific substrate and one without—transcriptomic studies can indicate which proteins play a role in the metabolic pathway of the...
19 July 2024 8,402 3 View
Dear community, I have bacteria that perform the first step of denitrification, so they reduce nitrate to nitrite. Nitrite accumulates to 1.5 mM in my culture. Then the bacteria stop being active....
22 October 2021 6,384 6 View
I was about to design the primers for SLiCE, when I realized the insert is so small I could order it as one oligo and it would not even be that expansive. As its quite some time ago, that I used...
19 March 2020 3,274 3 View
Dear research community, I am confused with the formatting of cytochromes. I will ask my question with cytochrome c, as an example. What is the most common abbreviation, cytc, cyt c or Cyc c? Is...
22 July 2019 1,966 2 View
I plan to follow a protocol that we use in our lab for protein extraction, however I am afraid to lose proteins in the first steps. We start with frozen cell pellets of gram negative bacteria...
11 February 2019 7,106 6 View
I am looking for a "not too old" review or paper on the regulation of NR or in general denitrification in bacteria. In particular I like to find an answer on how nitrite accumulation could inhibit...
01 January 1970 3,505 0 View
