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Questions related from Stefania Pezzana
Hi everybody! I´m trying to improve my protocol for CD4 T-cell expansion isolated from murine spleen and lymphnodes. Do you have any suggestion? Thank you a lot!
07 July 2019 7,763 8 View
Hi! I´m trying to set up a protocol for the evaluation of the internalization of a radiolabelled antibody; all the protocols that I´ve found suggest to lysate the cells and then to measure the...
04 April 2019 1,351 11 View
Hi! I´m quite new with FACS measurements; my question is: when I adjust the voltage of FSC, SSC and my fluorochrome, they need to be all different? And for the fluorochrome there is a specific...
04 April 2019 6,516 2 View
Hi, I would like to make a viability staining using 7-AAD the day before the measurement; Is it possible to fix the cells? If yes, what do you suggest? Thank you!
04 April 2019 3,884 2 View
I'm working with B16F10cell line... But I have some problem with melanin production. Now (3 passages after thawing) cells are transparent and melanin is only in the medium.. do You know which...
07 July 2018 2,483 1 View
