Sreeram Chandra Murthy Peela

10 Questions 25 Answers 0 Followers

Questions related from Sreeram Chandra Murthy Peela

Which databases are available to extract data about gene expression, proteome and metabolome in bacteria?

Hi I am new to the field of systems biology, with a background in molecular biology and microbiology. I would like to deduce the proteome and interactome of bacterial pathogens based on the...

02 April 2018 4,640 6 View

How to estimate evolution of genes using mega?

Hi all, I would like to estimate the relative rates of evolution of a bacterial gene locus (protein-coding) across time. The length of the locus ranges between 10000bp and 25000bp. I have...

27 November 2017 2,076 4 View

Which processor is better for running MD simulations - i7 7700 or AMD Ryzen 1700X?

Hi all, I wish to purchase an assembled PC for running MD simulations myself. My specifications are: 8GB RAM, 1 TB ROM, NVIDIA M2000 GPU apart from all other general features. I was unable to...

20 November 2017 1,547 8 View

How to find gi identifier in BLASTp for submitting y model to PMDB? Any databases other than PMDB for submitting modelled proteins?

Dear all, I have modelled a drug efflux protein using ITASSER. I would like to upload the model in Protein Model Database (http://www.biocomputing.it/PMDB). The accession number is...

19 October 2017 9,126 3 View

How to design primers if I want to sequence an entire gene?

Hi, I wish to sequence entire gene of Streptococcus pneumoniae. The length is 2.2 kb. I wish to detect presence of any mutations or variations in this gen. Can I design a single set of primers...

24 December 2016 1,863 5 View

Preparation of master stock of hydroxynaphthol blue dye for LAMP assay?

Hi, I have been trying to optimize LAMP assay using HNB dye (620.48 M.W.). However, I was unable to make any difference in color in the positive and negative controls. Please tell me the exact...

15 December 2016 3,769 4 View

High Cp values in qPCR?

I am working on detection of Streptococcus pneumoniae in clinical samples like blood and CSF. I have been doing the assay based on the primers and probe recommended by CDC. However I reduced the...

19 August 2016 9,083 8 View

Any web-based server program for protein dimerisation?

Hi, I am trying to predict the structure of a protein using homology modelling. I sucessfully predicted the protein structure. But the template submitted in PDB is a monomer. The active protein is...

22 July 2016 5,018 6 View

Is there any mathematical model describing the formation of galaxies?

I have been reading Feynman lectures for sometime. In the volume 1 he mentioned that no model at that time could explain the formation of galaxies. Is the problem still unanswered? Please provide...

09 July 2016 4,063 11 View

How to troubleshoot modeller 9.16 for structure prediction of my protein sequence?

hi. i have been trying to run Modeller 9.16 to predict structure of the protein. I created the necessary files (scripts) based on the examples provided in the tutorial. I just kept the default...

12 June 2016 4,142 3 View