12 Questions 28 Answers 0 Followers
Questions related from Sreeram Chandra Murthy Peela
Hi I am new to the field of systems biology, with a background in molecular biology and microbiology. I would like to deduce the proteome and interactome of bacterial pathogens based on the...
02 April 2018 4,692 6 View
Hi, I would like to derive a mathematical model that can explain the change in frequencies of two genes that cause resistance to a particular antibiotic. Based on the literature, one of the genes...
22 December 2017 845 1 View
Hi all, I would like to estimate the relative rates of evolution of a bacterial gene locus (protein-coding) across time. The length of the locus ranges between 10000bp and 25000bp. I have...
27 November 2017 2,139 4 View
Hi all, I wish to purchase an assembled PC for running MD simulations myself. My specifications are: 8GB RAM, 1 TB ROM, NVIDIA M2000 GPU apart from all other general features. I was unable to...
20 November 2017 1,620 8 View
Dear all, I have modelled a drug efflux protein using ITASSER. I would like to upload the model in Protein Model Database (http://www.biocomputing.it/PMDB). The accession number is...
19 October 2017 9,205 3 View
Hi, I would like to compare the biochemical and cytological values like cell count, glucose levels, etc of test samples between two different tests, PCR and culture, for identification of...
11 August 2017 6,263 6 View
Hi, I wish to sequence entire gene of Streptococcus pneumoniae. The length is 2.2 kb. I wish to detect presence of any mutations or variations in this gen. Can I design a single set of primers...
24 December 2016 1,940 5 View
Hi, I have been trying to optimize LAMP assay using HNB dye (620.48 M.W.). However, I was unable to make any difference in color in the positive and negative controls. Please tell me the exact...
15 December 2016 3,828 4 View
I am working on detection of Streptococcus pneumoniae in clinical samples like blood and CSF. I have been doing the assay based on the primers and probe recommended by CDC. However I reduced the...
19 August 2016 9,160 8 View
Hi, I am trying to predict the structure of a protein using homology modelling. I sucessfully predicted the protein structure. But the template submitted in PDB is a monomer. The active protein is...
22 July 2016 5,079 6 View
I have been reading Feynman lectures for sometime. In the volume 1 he mentioned that no model at that time could explain the formation of galaxies. Is the problem still unanswered? Please provide...
09 July 2016 4,163 11 View
hi. i have been trying to run Modeller 9.16 to predict structure of the protein. I created the necessary files (scripts) based on the examples provided in the tutorial. I just kept the default...
12 June 2016 4,239 3 View
