Sophie Irwin

6 Questions 11 Answers 0 Followers

Questions related from Sophie Irwin

Can circulating DNA in serum be used to match up serums at different time points to one patient?

I have been helping with a study looking at obesity. its a multiple timepoint study, so each patient should have 4 serums, baseline, 1 month, 3 months and 6 months. I have been processing the...

28 July 2022 1,547 0 View

Poly-Hema coming out of solution?

Hi, I'm doing a chemotaxis assay, and need to coat 24 well plates with Polyhema to prevent my neutrophils from sticking. I seem to be having a problem when I come to use the plate for the...

28 June 2016 5,537 4 View

How publishable is comparing PSTAT1 to beta actin?

Hi, I am looking at how IFNs induce pSTAT1 in neutrophils. I had a few issues picking up the STAT1 (I have only managed once with VERY concentrated protein, which is unrealistic in my experiments)...

05 February 2016 4,436 0 View

Does anyone use media/PBS for humidity in cell culture?

Hi All, I used media for humidity in my cell cutlure plate when incubating neutrophils, and one of the postdocs mentioned she's never heard of this. I've explained why, and humidity and 'have...

25 January 2016 4,587 12 View

Has anyone observed neutrophil stickiness with TNF alpha?

Hi all, I use TNF alpha to activate neutrophils in my experiments. I realised I've been using it too strong, however I've noticed the neutrophils always get 'sticky' after a half hour TNF alpha...

25 January 2016 7,223 2 View

How much FCS to add to neutrophils to stop them being too apoptotic?

Hi all, I'm currently incubating neutrophils for 6hrs, unstimulated and with IFN alpha, beta, gamma and gmcsf. I'm getting a lot of apoptosis (though less in my conditions) but this has been...

01 May 2015 6,633 8 View