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Questions related from Sophie Irwin
I am trying to isolate bacteria with and without bound IgA from oral washes using anti-IgA APC antibodies and anti-APC beads plus MACS columns. Both populations when run through the flow cytometer...
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I have been helping with a study looking at obesity. its a multiple timepoint study, so each patient should have 4 serums, baseline, 1 month, 3 months and 6 months. I have been processing the...
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Hi, I'm doing a chemotaxis assay, and need to coat 24 well plates with Polyhema to prevent my neutrophils from sticking. I seem to be having a problem when I come to use the plate for the...
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Hi, I am looking at how IFNs induce pSTAT1 in neutrophils. I had a few issues picking up the STAT1 (I have only managed once with VERY concentrated protein, which is unrealistic in my experiments)...
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Hi All, I used media for humidity in my cell cutlure plate when incubating neutrophils, and one of the postdocs mentioned she's never heard of this. I've explained why, and humidity and 'have...
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Hi all, I use TNF alpha to activate neutrophils in my experiments. I realised I've been using it too strong, however I've noticed the neutrophils always get 'sticky' after a half hour TNF alpha...
25 January 2016 7,258 2 View
I'm trying to analyse the expression of CD11b and CD62l on unstimulated and activated neutrophils. the CD62L works as expected, but the CD11b isn't doing what I'd expect - it works too well in an...
17 July 2015 8,710 5 View
Hi all, I'm currently incubating neutrophils for 6hrs, unstimulated and with IFN alpha, beta, gamma and gmcsf. I'm getting a lot of apoptosis (though less in my conditions) but this has been...
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