Sophie Briggs

5 Questions 15 Answers 0 Followers

Questions related from Sophie Briggs

What are the best concentration and timescales when pre-coating plates with Protamine Sulfate for DNA binding?

Hi all, I am using an ELISA method for detecting DNA and have established that Protamine Sulfate looks best for binding DNA to the plate. Does anyone have any suggestions for starting...

07 August 2018 2,506 1 View

Cell culture contamination with suspension cells?

I am culturing primary human brain stem cells which grow as neurospheres but I seem to constantly get what looks like bacterial contaminations and cannot seem to work out why. I have had 4 years...

04 May 2016 1,502 8 View

Does anyone know of a good antibody for TERT (human and/or mouse reactive)?

I am looking for an antibody for hTERT or mTERT but ideally reactive for both. The ones we have previously used are no longer being sold and we are struggling to find a decent one. Thanks

27 August 2015 8,911 3 View

Is it possible to get a stable, long term knockdown of ATRX in human or mouse fibroblasts?

I am just wondering if this is possible and all the publications I have come across appear to knockdown ATRX using siRNA or shRNA but they never report how long these knockdowns last. Any input...

18 August 2015 2,289 4 View

How can I correct unequal loading in Western Blots?

I am currently doing western blots with BTIC cells (Brain tumour initiating cells) which grow as neurospheres. I have done western blots before (with monolayer BTIC's) and had no issues...

23 June 2015 8,765 19 View