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Questions related from Siti Radziah
Below are the Component that I used in my ddPCR reaction Water 5uL MasterMix 12.5uL20x FAM 1.25uL20x VIC 1.25uLdigested DNA 6.6 ng/uL 5uL
28 March 2017 597 8 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as belowOnce i thawed can i stored back at -20C?-For the component as belowHow I should...
27 February 2017 3,698 2 View
Followed by 20minutes of heat inactivation at 65 degree C in a PCR thermal cycler. I dont understand? How to set up only heat inactivation? Because in PCR involve : Enzyme...
26 February 2017 5,937 3 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as below Once i thawed can i stored back at -20C?-For the component as below How I should...
25 February 2017 634 22 View
Suppose I pipette the buffy coat sample in to 2mL centrifuge tube containing 1.3mL of RNAlater? However I have collected and prepare the sample. I just realized that I did not include the...
05 February 2017 5,898 4 View
In which order I should add the following component for PCR reaction using ddPCR? Water 2x MasterMix 20x FAM 20x VIC digested DNA
31 January 2017 5,259 3 View
