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Questions related from Siti Radziah
What need to do if more than one population of positive droplets and negative droplets appear during analyzing the result by using Quanta Soft-software?
01 May 2017 3,925 1 View
Suppose the copy number variation is 2(normal),more or less than 2abnormal. In my study,the cnv is 7,6,5,4.why i am not getting 2?
19 April 2017 6,490 3 View
Anyone know what is the minimum concentration (copies/uL) that should obtained?
04 April 2017 750 1 View
Below are the Component that I used in my ddPCR reaction Water 5uL MasterMix 12.5uL20x FAM 1.25uL20x VIC 1.25uLdigested DNA 6.6 ng/uL 5uL
28 March 2017 627 8 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as belowOnce i thawed can i stored back at -20C?-For the component as belowHow I should...
27 February 2017 3,768 2 View
Followed by 20minutes of heat inactivation at 65 degree C in a PCR thermal cycler. I dont understand? How to set up only heat inactivation? Because in PCR involve : Enzyme...
26 February 2017 5,986 3 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as below Once i thawed can i stored back at -20C?-For the component as below How I should...
25 February 2017 709 22 View
If I ordered this RE EcoRV (R6351), does it come in the liquid form (diluted) or what form it will be arriving? What does it mean the components.., it's already diluted or the components will be...
18 February 2017 1,153 4 View
Suppose I pipette the buffy coat sample in to 2mL centrifuge tube containing 1.3mL of RNAlater? However I have collected and prepare the sample. I just realized that I did not include the...
05 February 2017 5,926 4 View
In which order I should add the following component for PCR reaction using ddPCR? Water 2x MasterMix 20x FAM 20x VIC digested DNA
31 January 2017 5,421 3 View