Sindhushree Raghunandan

5 Questions 9 Answers 0 Followers

Questions related from Sindhushree Raghunandan

Statistical Analysis of Agilent Seahorse MitoStress Test Data

I have three biological repeats of my experiment but the variance across the experiments is very high (although the trend among my treatment groups in each experiment is consistent). Does anyone...

08 August 2017 8,407 3 View

Is it possible to quantify mitochondrial RNA by qPCR?

If so, does anyone have recommendations for mito-RNA primers specific for the ETC complex subunits?

03 March 2017 3,089 15 View

Anyone know an image analysis protocol to identify the number of blue stained cells in a phase contrast image with a fair amount of background signal?

I'm working with images of cells stained for beta-galactosidase but the blue signal is not very prominent compared to the amount of gray/background signal. 1. what methods do you use for...

08 August 2015 3,329 8 View

Will pre-treating petri dishes with collagen or gelatin affect HUVEC gene expression?

I'm trying to interface between two phases of a project - the first set of transcriptomic measurements were collected from cells plated on dishes that were not pre-treated. The next phase involves...

03 March 2015 6,477 8 View

Is there a way to quantify the branchedness of mitochondrial networks in a stack of 2D fluorescence microscopy images?

More specifically - I'm looking for something that will: construct an approximate 3D model of the nucleus and surrounding mitochondrial network from the stack of 2D images (where the nucleus and...

12 December 2014 3,468 4 View