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Questions related from Simona Di Blasio
LAPT broth: 1.5% Bacto Peptone, 1% Bacto Tryptone, 1% yeast extract, 1% glucose and 0.1% Tween 80
08 November 2023 8,201 2 View
I am trying to grow L. amylovorus NCIMB 702661 So far I managed to make it grow on MRS agar in aerobic and anaerobic condition (very small and thin colonies) I am still having problems with...
23 February 2022 8,799 1 View
I am having problems with solubilising a low MW chitosan (MW ~15kDa). I tried to solubilise 25 mg in 5 ml of 1% acetic acid under stirring, but the solution remained very cloudy and precipitate...
24 March 2020 7,779 6 View
I need to develop a method to detect the mass of an oligonucleotide 10kDa using Microflex LT system Bruker Daltonik and Flex control sofware. The programme has already some methods, such as LN...
31 October 2017 7,031 8 View
My molecule molecular weight is 564.86, the chemical formula is C38H52N42+. The positive charges are on the nitrogens. I ran a mass spec to characterise it and I found 281.9 as major peak. 290 and...
17 May 2016 2,334 1 View
I tried with H2(g)/Pd on Carbon 10% wt at atmospheric pressure (balloon) but it did not work. Then I tried with ammonium formate and Pd/C at room temperature but it did not work either. I was...
01 April 2016 9,208 8 View
I am using a Dowex 1x4 chloride form resin to do an ion-exchange. I am following a protocol where the ion exchange is performed in a beaker under stirring. First of all I wash the resin with...
15 October 2015 8,145 2 View
I added BF3Et2O to my reaction mixture and it became very gluey.
22 April 2015 5,194 4 View
