Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How to calculate enthalpy of formation of Polymers taking into account the terminal end groups?

I am trying to find methods for theoretical calculation of enthalpy of formation of Polymers. till now I have found the oligomeric method (Gaussian) which uses the extrapolation of results from...

05 February 2021 9,707 4 View

Can any tell me or suggest any research article or review about the concentration of peptide dissolve in system water and given to Adult Zebrafish?

Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...

01 February 2021 5,809 3 View

How can we polymerize carbon to form a layer? Any easy method is there?

I want to polymerize the carbon to make a thin sheet. Is there any easy method to polymerize it?

10 December 2020 1,762 4 View

I am designing an UWB antenna..Please guide how to get wideband instead of multiband..?

By slotting patch and ground I am getting multiband not wideband.pls guide

09 November 2020 3,924 4 View

What is the best way to learn materials simulation using MATLAB?

I want to study the electronic, optical or catalytic properties of two-dimensional materials using MATLAB. What is the best way to start learning?

26 October 2020 1,821 8 View

Can I run joint wald test for more than two regressions to interpret whether the intercepts are jointly equal to zero or not?

I have run 10 regressions using same model for a sample by diving the the sample on the basis of some criteria. The problem I am facing is that I can test the intercepts for individual sample...

25 September 2020 6,314 3 View

Is Cronbach's alfa value 0.5 acceptable ?

I am calculating the whistleblower policy disclosure score for 100 Listed firms in India. I took 31 questions under four categories and scored them 0, 1 & 2 on three-level a) 2 points: If...

09 June 2020 7,729 3 View

My Work Is On Information Retrieval Ranking Using Pso+Svm ,Using Trec Dataset Of Q/A ,But Some Good Journals Rejected Commenting Its Basic,Is It?

I did my implementation in python and represented my result score result of answers of the questions.I represented graph for all the evaluation parameters and confusion matrix,why i am not able to...

24 March 2020 4,235 3 View

What are the statistical method or procedure to construct Index?

I am constructing the whistleblower policy Disclosure Index for the top 100 listed companies in India. I have taken 31 attributes/questions/parameters under four major categories i.e. Board...

23 March 2020 6,571 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View