Shubhra Sachan

2 Questions 3 Answers 0 Followers

Questions related from Shubhra Sachan

Why is only the gene specific primer giving the positive result and not the t7 primers?

At first when I started colony cloning a 270 BP insert into 5677 BP vector( pet sumo adapt) I had a lot of problems in confirming my positive clone using colony pcr. I did colony pcr with t7...

01 June 2020 9,499 4 View

Why is ligation reaction not giving the plasmid of interest when seen on 1.5 percent agarose gel?

I have used pet sumo vector, restriction enzymes: bsaI and BamHI, double digestion. ligation reaction mix : vector (150ng), insert (750ng) total Ligation volume 20 uL plate 1 : 20ul of ligation...

20 May 2020 8,323 7 View